pirfp670 n1 (Addgene inc)
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Addgene inc
pirfp670 n1
Pirfp670 N1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pirfp670+n1/piRFP670-N1+(Plasmid+%2345457)/bio_rxiv__64898__2026__02__23__707339-169-11-18
Average 93 stars, based on 86 article reviews
Pirfp670 N1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pirfp670+n1/piRFP670-N1+(Plasmid+%2345457)/bio_rxiv__64898__2026__02__23__707339-169-11-18
Average 93 stars, based on 86 article reviews
pirfp670 n1 - by Bioz Stars,
2026-09
93/100 stars
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Construct:Article Title: Systems and methods for determining a treatment course of action Article Snippet: Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and mRuby2-N1 (a gift from Michael Davidson, Addgene #54614) to create C-terminal tagged MRLC. .. Fusion construct was subcloned into the expression vector pCIG3 (pCMVIRES-GFP, a gift from Felicia Goodrum, Addgene plasmid #78264), modified to replace the GFP cassette with puromycin resistance gene. pCMV-iRFP670-EVL-IRES-BlastR was generated by PCR amplification of cDNA for Mus musculus EVL cDNA from MSCV-eGFP-EVL and Article Title: A Reporter Platform to Study Therapy‐Induced Senescence in Live Cancer Cells Article Snippet: .. Dronpa‐LaminB1‐10 was a gift from Michael Davidson, used for the creation of the SRbr, SRbfr, SRsh, SRf and SRs constructs (Addgene plasmid #57 282); mCherry‐LaminA‐C‐18 was a gift from Michael Davidson (Addgene plasmid #55 068); pHRM‐NLS‐dCas9‐GFP11×7‐NLS‐P2A‐BFP‐dWPRE was a gift from Bo Huang for the creation of the SRs construct (Addgene plasmid #70 224) [ ] ; Article Title: A Reporter Platform to Study Therapy-Induced Senescence in Live Cancer Cells. Article Snippet: .. Dronpa-LaminB1-10 was a gift from Michael Davidson, used for the creation of the SRbr, SRbfr, SRsh, SRf and SRs constructs (Addgene plasmid #57 282); mCherry-LaminA-C-18 was a gift from Michael Davidson (Addgene plasmid #55 068); pHRM-NLS-dCas9-GFP11×7NLS-P2A-BFP-dWPRE was a gift from Bo Huang for the creation of the SRs construct (Addgene plasmid #70 224)[59]; Expressing:Article Title: Systems and methods for determining a treatment course of action Article Snippet: Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and mRuby2-N1 (a gift from Michael Davidson, Addgene #54614) to create C-terminal tagged MRLC. .. Fusion construct was subcloned into the expression vector pCIG3 (pCMVIRES-GFP, a gift from Felicia Goodrum, Addgene plasmid #78264), modified to replace the GFP cassette with puromycin resistance gene. pCMV-iRFP670-EVL-IRES-BlastR was generated by PCR amplification of cDNA for Mus musculus EVL cDNA from MSCV-eGFP-EVL and Article Title: Supporting Information for Visualization of trans homophilic interaction of clustered protocadherin in neurons Article Snippet: .. The expression vector for iRFP670 was subcloned from Article Title: Native receptor-targeted chemogenetics enables cell-type-specific inhibition of endogenous receptors in freely moving mice Article Snippet: .. HEK293 cells were co-transfected with an expression plasmid encoding mGlu1 and Plasmid Preparation:Article Title: Systems and methods for determining a treatment course of action Article Snippet: Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and mRuby2-N1 (a gift from Michael Davidson, Addgene #54614) to create C-terminal tagged MRLC. .. Fusion construct was subcloned into the expression vector pCIG3 (pCMVIRES-GFP, a gift from Felicia Goodrum, Addgene plasmid #78264), modified to replace the GFP cassette with puromycin resistance gene. pCMV-iRFP670-EVL-IRES-BlastR was generated by PCR amplification of cDNA for Mus musculus EVL cDNA from MSCV-eGFP-EVL and Article Title: Supporting Information for Visualization of trans homophilic interaction of clustered protocadherin in neurons Article Snippet: .. The expression vector for iRFP670 was subcloned from Article Title: A Reporter Platform to Study Therapy‐Induced Senescence in Live Cancer Cells Article Snippet: .. Dronpa‐LaminB1‐10 was a gift from Michael Davidson, used for the creation of the SRbr, SRbfr, SRsh, SRf and SRs constructs (Addgene plasmid #57 282); mCherry‐LaminA‐C‐18 was a gift from Michael Davidson (Addgene plasmid #55 068); pHRM‐NLS‐dCas9‐GFP11×7‐NLS‐P2A‐BFP‐dWPRE was a gift from Bo Huang for the creation of the SRs construct (Addgene plasmid #70 224) [ ] ; Article Title: Native receptor-targeted chemogenetics enables cell-type-specific inhibition of endogenous receptors in freely moving mice Article Snippet: .. HEK293 cells were co-transfected with an expression plasmid encoding mGlu1 and Article Title: MicroRNA-responsive ON-OFF hybrid mRNA switch for precise protein expression control Article Snippet: PCR products were cloned into PCR-linearized pUC19 vector by Gibson assembly using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, no. E2621) and transformed to DynaCompetent Cells JetGiga E. coli DH5α (BioDynamics Laboratory, no. DS230). .. After culturing E. coli at 37°C for 16 h with 3 mL LB/ampicillin (Amp) media, plasmids were purified by the PureYield Plasmid Miniprep System (Promega, no. A1223). Article Title: A Reporter Platform to Study Therapy-Induced Senescence in Live Cancer Cells. Article Snippet: .. Dronpa-LaminB1-10 was a gift from Michael Davidson, used for the creation of the SRbr, SRbfr, SRsh, SRf and SRs constructs (Addgene plasmid #57 282); mCherry-LaminA-C-18 was a gift from Michael Davidson (Addgene plasmid #55 068); pHRM-NLS-dCas9-GFP11×7NLS-P2A-BFP-dWPRE was a gift from Bo Huang for the creation of the SRs construct (Addgene plasmid #70 224)[59]; Article Title: Novel Kinesin Family Member 1A Variants Linked to Atypical Parkinsonism Elicit Altered Neuronal Transactive Response DNA Binding Protein 43 kDa Interactions and Dendritic Atrophy Article Snippet: The human synapsin I promoter (hSyn) was PCR amplified from pLV-hSyn-RFP (Addgene, Watertown, MA; catalog number 22909). .. The fragment was then inserted into Modification:Article Title: Systems and methods for determining a treatment course of action Article Snippet: Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and mRuby2-N1 (a gift from Michael Davidson, Addgene #54614) to create C-terminal tagged MRLC. .. Fusion construct was subcloned into the expression vector pCIG3 (pCMVIRES-GFP, a gift from Felicia Goodrum, Addgene plasmid #78264), modified to replace the GFP cassette with puromycin resistance gene. pCMV-iRFP670-EVL-IRES-BlastR was generated by PCR amplification of cDNA for Mus musculus EVL cDNA from MSCV-eGFP-EVL and Generated:Article Title: Systems and methods for determining a treatment course of action Article Snippet: Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and mRuby2-N1 (a gift from Michael Davidson, Addgene #54614) to create C-terminal tagged MRLC. .. Fusion construct was subcloned into the expression vector pCIG3 (pCMVIRES-GFP, a gift from Felicia Goodrum, Addgene plasmid #78264), modified to replace the GFP cassette with puromycin resistance gene. pCMV-iRFP670-EVL-IRES-BlastR was generated by PCR amplification of cDNA for Mus musculus EVL cDNA from MSCV-eGFP-EVL and Polymerase Chain Reaction:Article Title: Systems and methods for determining a treatment course of action Article Snippet: Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and mRuby2-N1 (a gift from Michael Davidson, Addgene #54614) to create C-terminal tagged MRLC. .. Fusion construct was subcloned into the expression vector pCIG3 (pCMVIRES-GFP, a gift from Felicia Goodrum, Addgene plasmid #78264), modified to replace the GFP cassette with puromycin resistance gene. pCMV-iRFP670-EVL-IRES-BlastR was generated by PCR amplification of cDNA for Mus musculus EVL cDNA from MSCV-eGFP-EVL and Amplification:Article Title: Systems and methods for determining a treatment course of action Article Snippet: Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and mRuby2-N1 (a gift from Michael Davidson, Addgene #54614) to create C-terminal tagged MRLC. .. Fusion construct was subcloned into the expression vector pCIG3 (pCMVIRES-GFP, a gift from Felicia Goodrum, Addgene plasmid #78264), modified to replace the GFP cassette with puromycin resistance gene. pCMV-iRFP670-EVL-IRES-BlastR was generated by PCR amplification of cDNA for Mus musculus EVL cDNA from MSCV-eGFP-EVL and Transfection:Article Title: Native receptor-targeted chemogenetics enables cell-type-specific inhibition of endogenous receptors in freely moving mice Article Snippet: .. HEK293 cells were co-transfected with an expression plasmid encoding mGlu1 and Marker:Article Title: Native receptor-targeted chemogenetics enables cell-type-specific inhibition of endogenous receptors in freely moving mice Article Snippet: .. HEK293 cells were co-transfected with an expression plasmid encoding mGlu1 and Purification:Article Title: MicroRNA-responsive ON-OFF hybrid mRNA switch for precise protein expression control Article Snippet: PCR products were cloned into PCR-linearized pUC19 vector by Gibson assembly using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, no. E2621) and transformed to DynaCompetent Cells JetGiga E. coli DH5α (BioDynamics Laboratory, no. DS230). .. After culturing E. coli at 37°C for 16 h with 3 mL LB/ampicillin (Amp) media, plasmids were purified by the PureYield Plasmid Miniprep System (Promega, no. A1223). |